ATAC-Seq Maytum et al. VEGF study hg38 28 tracks

Chromatin accessibility across iPSC to HSPC differentiation

Twenty-eight ATAC-Seq tracks spanning day 0 to day 18, with and without VEGF. Load them into the UCSC Genome Browser in one click. No account needed.

Loading the hub

  1. 01
    Open the hub

    This link attaches the hub and takes you straight to the browser.

    Open in UCSC Genome Browser ↗
  2. 02
    Or add it by hand

    Go to My Hubs on UCSC, paste this into the URL box, and click Add Hub.

    https://object-store.rc.nectar.org.au/v1/AUTH_e9f87ff834264c90967178b4c61ebe05/renew-blood-atac-hg38/hub.txt

    Assembly must be hg38. The tracks will not appear on hg19.

What loads

Tracks appear in a group called Maytum VEGF ATAC, below the standard UCSC tracks. Days 0 to 7 are an unbranched timecourse; from day 8 the series splits into +VEGF and −VEGF across four populations.

D0
iPSC
1 track
D1
Primitive streak
1 track
D3
Mesoderm
1 track
D5–D12
Arterial endothelium
9 tracks
D8–D12
Haemogenic endothelium
6 tracks
D10–D12
EaB
4 tracks
D14–D18
HSPC
6 tracks

Getting the most from it

Use the matrix, not the checkboxes. Click the V comparison D8-D18 title to open a grid with days down the side and +V / −V across the top. Tick a row or a column instead of toggling 22 tracks one at a time.

Overlay tracks show the VEGF effect directly. Find one named like D10 Art.Endo V and set it to full. It draws +VEGF and −VEGF superimposed on shared axes in a single row.

The y-axis is fixed at 0 to 10, deliberately. These tracks are depth-normalised, so autoscaling is off and equal track height means equal signal. Heights are directly comparable between any two tracks.

Where to look first

Try a haematopoietic locus. Developmental genes outside the blood lineage will look flat, which is correct rather than a loading problem.

RUNX1 GATA2 SPI1 CD34 TAL1 SHH